Session A8 - Focus Session: Neutron Scattering and Other Studies of Phospholipids.
FOCUS session, Monday morning, March 22
510A, Palais des Congres
Supported phospholipid membranes (lipid bilayers and monolayers) on solids have proven to be useful model surfaces for the characterization of many important biological processes. Recently, we have reported a method of preparing a surface-grafted phospholipid monolayer using in situ polymerization at the interface between a mono-acrylated lipid monolayer and a methacryloyl-terminated surface. As confirmed by studies on water contact angles, atomic force microscopy, and X-ray photoelectron spectroscopy, the polymerized phospholipid monolayer (poly-PC) was chemically bonded to a solid in a substantial manner and it was very stable to organic solvents. The reduced adsorption of proteins and macrophase on the poly-PC were clearly observed by in vivo and in vitro experiment, respectively. For the potential application of a surface-grafted phospholipid monolayer, it is crucial to determine the detailed physical structure of the lipid monolayer grafted on solids. The studies are therefore concerned with a depth profiling measurement that characterizes the surface properties of bare and PEG covered phospholipid surfaces before and after in situ polymerization, where demonstrates that in situ polymerization techniques can produce a stabilized phospholipid monolayer surface. Part of this work was supported by the Korea Research Foundation.